Ganglioside GM1 localization in cholinergic neurons visualized by use of a double avidin-biotin complex system.
نویسندگان
چکیده
Localization of ganglioside GM1 in cholinergic neurons from the septal area of a primary culture newborn rat brain was studied with a double avidin-biotin complex system. Cholinergic neurons were identified by double immunolabeling techniques that use choline acetyltransferase (ChAT) and neurofilament (NF) protein-antibodies. ChAT-positive neurons also were stained for ganglioside GM1 by using an avidin-biotin complex technique.
منابع مشابه
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ورودعنوان ژورنال:
- Cell structure and function
دوره 11 2 شماره
صفحات -
تاریخ انتشار 1986